
Cat parentity testing
Cat parentity testing
Are you interested in yours kitten pedigree? Is your kitten really offspring of the parents, you chose according to their accomplishments? Are you not sure which sire is father yours dam litter? Cat parentage testing and DNA identification can help you to answer these questions.
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For high probability of cat parentage testing is necessary to analyze samples of both parents - because cats of the same breed are very similar and differ only in a few markers.
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DNA profile can be determined from a blood sample or a cheek swabs. Cat paternity analysis is the same as with humans. Common markers among offspring DNA and parents DNA are searched for. A parentage is proved, if probable parent is detected like biological parent. Sequences repeated in tandems (microsatellites) are used for parentage testing. These microsatellites contain sequences repeated in tandems from two to seven base pairs. Microsatellites are inherited in Mendelian manner. Each offspring has to correspond with a parent in 50% of each microsatellite marker. Microsatellite markers are used for individual identification and, for paternity analysis in humans and animals. Size of a marker (number of base pair in a microsatellite) is detected by polymerase chain reaction (PCR) and subsequent fragment analysis. One of the primers for PCR is marked on 5´end by a florescent dye. Fragments are separated and detected by automatic electrophoresis instrument.
For cat parentage testing following loci are used FCA069, FCA075, FCA105, FCA149, FCA220, FCA229, FCA310, FCA441, FCA678, Amelogenin, FCA026, FCA201, FCA293, FCA453, FCA649. These markers are recommended by the International Society of Animal Genetics (ISAG).
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Examination is performed by partner lab (accredited according ISO 17025).
Price for each test is 47,9 Eur. (Father + mother + child = 143,7 Eur).


