Dog parentity testing

Dog paternity testing

Are you interested in yours puppy pedigree? Is your puppy really offspring of the parents, you chose according to their accomplishments? Are you not sure which male-dog is father yours female-dog litter? Dogs parentage testing and DNA identification can help you to answer these questions.

For high probability of dog parentage testing is necessary to analyze samples of both parents - because dogs of the same breed are very similar and differ only in a few markers.

DNA profile can be determined from a blood sample. Dog paternity analysis is the same as with humans. Common markers among offspring DNA and parents DNA are searched for. A parentage is proved, if probable parent is detected like biological parent. Sequences repeated in tandems (microsatellites) are used for parentage testing. These microsatellites contain sequences repeated in tandems from two to seven base pairs. Microsatellites are inherited in Mendelian manner. Each offspring has to correspond with a parent in 50% of each microsatellite marker. Microsatellite markers are used for individual identification and, for paternity analysis in humans and animals. Size of a marker (number of base pair in a microsatellite) is detected by polymerase chain reaction (PCR) and subsequent fragment analysis. One of the primers for PCR is marked on 5´end by a florescent dye. Fragments are separated and detected by automatic electrophoresis instrument.
For dog parentage testing at least 14 of these 19 locuses are used AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, Amelogenin, FH2848, AHT121, FH2054, REN162C04 AHTh171 a REN247M23. These markers are recommended by the International Society of Animal Genetics (ISAG).