How to avoid a cross-reactivity during allergy test

New IgE- specific testing for dogs and cats allergies - screening and blocking of cross-reactivity

The results of allergy tests are more accurate, now :-)

NEXT+ presents a new generation of test to determine allergen specific IgE antibodies in canine and feline sera. It uses monoclonal antibodies against IgE generated from recombinant canine IgE to eliminate contamination with IgG or other immunoglobulins. Thanks to this, NEXT + maintains 100% specificity for IgE.

Completely innovative is its ability to block cross-reactive carbohydrate determinants (CCD). What does it mean? Most proteins associated with allergic diseases are glycoproteins. Besides allergenic proteins, their structure also contains carbohydrates.  CCDs are considered the most abundant allergenic structure in nature. They form a part of many plant allergens, such as pollen and vegetable food. Some of these carbohydrates are able to induce immune response during which specific IgE antibodies against the carbohydrate chain are produces and may affect the test results for presence of IgE. This phenomenon occurs in 30 % of human, canine, and feline population.

NEXT+ test is based on detection and blocking of specific IgE against cross-reactive carbohydrate determinants (CCDs). CCD screening is carried out before the test. In case of positive finding, 100% specific semisynthetic blocker is added to the test which is responsible for blocking the linkage between IgE and CCD. Therefore, the results obtained do not contain anti-CCD IgE. There are detected only allergens that are really involved in the allergic disease. NEXT+ eliminates false positive results obtained due to generation of anti-CCD IgE. The specificity and sensitivity of the test is significantly increased. The CCD-blocking during the in vitro test increased the correlation with intradermal tests and achieved higher clinical specificity of in vitro tests. The results are more specific, more accurate and more helpful in suggesting a suitable immunotherapy, the only etiologic treatment of allergic disease.