
Birds Pigeon - genetic profile
Determination of genetic profile - Pigeon
For parentage verification, please, order following test for the offspring: Pigeon - parentage to the genetic profile - for young
For testing
a genetic profile blood samples are preferred. The analysis is very
sensitive to DNA-quality. For the analysis is sufficient 2 drops of
blood in a tube with ethanol.
Profile
determination can also be done from blood spots or plucked feathers
(minimum 7 pieces).
The determination of genetic profile means determination of a set of genetic markers that are characteristic and unmistakable for the given individual (such as, for example, fingerprints of people). On the basis of a genetic profile, a Accipiter can be uniquely identified.
To determine the genetic
profile, tandem repetitive sequences (STR) are used. These sequences,
also called microsatellites, are a class of genetic markers
consisting of tandem sequence repeats with a size of two to seven
base pairs. The alleles of STR locus change according to the number
of repeats of the given sequence motive. Due to high level of
polymorphism (informativity) and Mendelian inheritance, the
microsattelites became suitable genetic markers for the study of
diversity, parentage tests and the genetic identity.
STR
alleles are determined by means of multiplex polymerase chain
reaction (multiplex PCR). One of the primers of each pair is at
5´-end labelled with a fluorescent dye. Fragments are divided and
detected in only one electrophoretic probe using an automatic
electrophoresis device.
The laboratory uses for Pigeon DNA profile determination following markers: CliuD11, PIGN15, CliMT43,, PIGN10, CliMD01, PIGN57, CliMT13, PIGN26, CliMD16, CliMD19, PIGN12, CliuMT02, CliMD17, CliMD35, CliMT17, PIGN04
.
Reference:
M. de Groot and W. A. van Haeringen: An evaluation of the International Society for Animal Genetics recommended parentage and identification panel for the domestic pigeon (Columba livia domestica), 2017 VHLGenetics, Agro Business Park 100, NL 6708 PW, Wageningen, The Netherlands