Determination of genetic profile - Haliaeetus albicilla

For testing a genetic profile blood samples are preferred. The analysis is very sensitive to DNA-quality. For the analysis is sufficient 2 drops of blood in a tube with ethanol.

The determination of genetic profile means determination of a set of genetic markers that are characteristic and unmistakable for the given individual (such as, for example, fingerprints of people). On the basis of a genetic profile, a Accipiter can be uniquely identified.

To determine the genetic profile, tandem repetitive sequences (STR) are used. These sequences, also called microsatellites, are a class of genetic markers consisting of tandem sequence repeats with a size of two to seven base pairs. The alleles of STR locus change according to the number of repeats of the given sequence motive. Due to high level of polymorphism (informativity) and Mendelian inheritance, the microsattelites became suitable genetic markers for the study of diversity, parentage tests and the genetic identity.
STR alleles are determined by means of multiplex polymerase chain reaction (multiplex PCR). One of the primers of each pair is at 5´-end labelled with a fluorescent dye. Fragments are divided and detected in only one electrophoretic probe using an automatic electrophoresis device.

Tested markers: AG12, Aa49, AG04, Aa27, AG05.

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Reference:

Literák, I., Mrlík, V., Hovorková, A. et al. Origin and genetic structure of white-tailed sea eagles (Haliaeetus albicilla) in the Czech Republic: an analysis of breeding distribution, ringing data and DNA microsatellites. Eur J Wildl Res 53, 195–203 (2007). https://doi.org/10.1007/s10344-006-0081-z